The Rotherhithe Tunnel is a road tunnel crossing beneath the River Thames in East London. It connects the Ratcliff district of Limehouse in the London Borough of Tower Hamlets north of the river to Rotherhithe in the London Borough of Southwark south of the river. It is designated as the A101. It was formally opened in 1908 by George Prince of Wales.

The work took place between 1904 and 1908, executed by the resident engineer Edward H. Tabor and the contractors Price and Reeves at a cost of about £1 million.

The Rotherhithe Tunnel consists of a single bore, 4,860 feet (1,481 m) long, carrying a two-lane carriageway 48 feet (14.5 m) below the high-water level of the Thames, with a maximum depth of 75 feet (23 m) below the surface.